New PDF release: Agrobacterium Protocols

By Kan Wang

ISBN-10: 1588298434

ISBN-13: 9781588298430

ISBN-10: 1597451312

ISBN-13: 9781597451314

Agrobacterium tumefaciens is a soil bacterium that for greater than a century has been referred to as a pathogen inflicting the plant crown gall ailment. not like many different pathogens, Agrobacterium has the facility to convey DNA to plant cells and completely adjust the plant genome. the invention of this specific characteristic 30 years in the past has supplied plant scientists with a robust instrument to genetically rework crops for either uncomplicated study reasons and for agric- tural improvement. in comparison to actual transformation equipment comparable to particle bomba- ment or electroporation, Agrobacterium-mediated DNA supply has a number of benefits. one of many positive aspects is its propensity to generate unmarried or a low reproduction variety of built-in transgenes with outlined ends. Integration of a unmarried transgene replica into the plant genome is much less prone to set off “gene silencing” frequently linked to a number of gene insertions. whilst the 1st version of Agrobacterium Protocols used to be released in 1995, just a handful of vegetation may be usually remodeled utilizing Agrobacterium. Ag- bacterium-mediated transformation is now standard to introduce DNA into many plant species, together with monocotyledon crop species that have been formerly thought of non-hosts for Agrobacterium. such a lot notable are contemporary devel- ments indicating that Agrobacterium can be used to bring DNA to non-plant species together with micro organism, fungi, or even mammalian cells.

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Mol. Res. 2, 334–347. 5. , et al. (1998) Pinpointing towards improved transformation and regeneration of cassava (Manihot esculenta Crantz). Plant Sci. 135, 87–101. 6. Puonti-Kaerlas, J. (1998) Cassava biotechnology. Biotechnol. Genet. Revs. 15, 329–364. 7. , and Zhang, P. (2004) Development and application of transgenic technologies in cassava. Plant Mol. Biol. 56, 671–688. 8. , and Puonti-Kaerlas, J. (2000) Production of stably transformed cassava plants via particle bombardment. Plant Cell Rep.

From 4E medium, shoots are excised and cultured on rooting medium (17N). 7. Root formation takes approx 2–3 wk after which the plants are transplanted to soil. In vitro grown plants maintained on 4E medium are used for transgene analysis. 5. Greenhouse Care of Transgenic Plants 1. Transgenic plants that have been grown in vitro for 1 mo are transferred to sterile soil (3:1 soil/vermiculite) and grown in the greenhouse under natural lighting conditions and a temperature range of 22–30°C. The transferred plants are covered with transparent plastic bags to maintain high humidity.

CIAT Working Document 123, 222–228. , and Marks, J. (2002) Influence of explant source, and light on efficiency of Agrobacterium-mediated transformation of cassava. Fifth International Meeting of the Cassava Biotechnology Network. St. , USA. November 4–9. , and Fauquet, C. (1997) An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and auxiliary bud-derived meristems. Plant Cell Rep. 16, 444–449. , and Potrykus, I. (1997) Development of a meristem gene transfer technique for cassava.

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Agrobacterium Protocols by Kan Wang


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