By Henry Y. Wang and Tadayuki Imanaka (Eds.)
content material: construction of engineered antibodies in myeloma and hybridoma cells : improvements in gene expression and technique layout / D.K. Robinson, D. DiStefano, S.L. Gould, G. Cuca, T.C. Seamans, D. Benincasa, S. Munshi, C.P. Chan, J. Stafford-Hollis, G.F. Hollis, D. Jain, ok. Ramasubramanyan, G.E. Mark, and M. Siberklang --
Antibody construction in human hybridomas with growth-associated creation kinetics / Hidekazu Sawada and Kazuaki Kitano --
Antibody creation in chinese language hamster ovary cells utilizing an impaired selectable marker / R.S. Barnett, K.L. Limoli, T.B. Huynh, E.A. Ople, and M.E. Reff --
Antibody construction in insect cells / Kathleen N. Potter, Yucheng Li, and J. Donald Capra --
Antibody expression in crops / J. K-C. Ma --
Engineering of immunoglobulin Fc and single-chain Fv proteins in Escherichia coli / David Filpula, Michele Rollence, Nina Essig, James Nagle, Aniruddha Achari, and Timothy Lee --
T-cell particular immunofusion proteins from Escherichia coli : motive, layout, and homes / Marc higher and Patricia A. Nolan --
creation and purification of antibody utilizing Bacillus subtilis as an expression host / S.L. Wong, X.C. Wu, L.P. Yang, S.C. Ng, and N. Hudson --
Trichoderma reesei, a promising novel host for antibody construction / Eini Nyyssönen and Sirkka Keränen --
utilising antibody catalysis to natural synthesis / Brian J. Lavey and Kim D. Janda --
powerful and particular peroxidase task with an antibody L chain-porphyrin Fe(III) advanced / Tadayuki Imanaka and Masahiro Takagi.
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Additional resources for Antibody Expression and Engineering
Perfusion was begun from the fourth day of the culture. Reprinted from Sawada et. , (1995) by permission of springer-Verlag GmbH & Co. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1995. ch002 24 ANTIBODY EXPRESSION AND ENGINEERING 1 m 2 m 3 Figure 5: SDS-PAGE pattern in reducing conditions of anti-HBsAb human MoAbs. Lane 1: HBW4, Lane 2: W471, Lane 3: HBW6, m: marker Neutralizing Activity of Human MoAbs Neutralizing tests of anti-HBsAg human MoAbs were performed with chimpanzees at the TNO Primate Center in the Netherlands.
Journal of Immunological Methods 1994, 167, 271-278. Page, M. ; Sydenham, M. A. Bio/Technology 1991, 9, 64-68. Fouser, L . ; Swanberg, S. ; Cumming, D. ; Riedel, G. E. Bio/Technology 1992, 10, 1121-1127. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1995. Chapter 4 Antibody Production in Insect Cells Kathleen N. Potter, Yucheng Li, and J. ch004 Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75235 The baculovirus expression system produces complete, biologically active human, mouse, human-mouse hybrid and human-mouse chimeric antibodies of the IgG, IgM and IgA isotypes in insect cells.
Natl. Acad. , 79, 1158-1162. 26. , and Kida, M. (1986). Production of human monoclonal antibodies by heterohybridomas. Appl. Microbiol. Biotechnol 24, 282-286. 27. , and Murakami, H. (1988) High cell density perfusion culture of hybridoma cells recycling high molecular weight components. Cytotechnology, 1, 171-175. 28. , and Kitano, K. (1991) Comparison of culture methods for human-human hybridomas secreting anti-HBsAg human monoclonal antibodies. Cytotechnology, 6, 197-208. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1995.
Antibody Expression and Engineering by Henry Y. Wang and Tadayuki Imanaka (Eds.)