Aqueous two-phase systems. Methods and protocols - download pdf or read online

By Rajni Hatti-Kaul

ISBN-10: 8906035411

ISBN-13: 9788906035418

A mix of polymers, or one polymer and a salt, in an aqueous medium separates into levels: this phenomenon comes in handy in biotechn- ogy for product separations. Separation of organic molecules and debris in those aqueous two-phase structures (ATPS) used to be initiated over forty years in the past via P.-Å. Albertsson, and later proved to be of giant software in biochemical and telephone organic study. a lift within the software of ATPS was once visible while difficulties of separations in biotechnology procedures have been encountered. Its simplicity, biocompatibility, and amenability to effortless scaleup operations make using ATPS very beautiful for large-scale bioseparations. regardless of the benefits ATPS enjoys over different separation suggestions, the applying of two-phase platforms has for a very long time been limited to chose labora- ries. fresh years have, besides the fact that, proven a development during which expanding numbers of researchers hire two-phase partitioning strategies in either easy and utilized learn.

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Download PDF by Rajni Hatti-Kaul: Aqueous two-phase systems. Methods and protocols

A mix of polymers, or one polymer and a salt, in an aqueous medium separates into levels: this phenomenon comes in handy in biotechn- ogy for product separations. Separation of organic molecules and debris in those aqueous two-phase platforms (ATPS) was once initiated over forty years in the past by means of P. -Å.

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Extra resources for Aqueous two-phase systems. Methods and protocols

Example text

The chamber is illuminated with a Nikon fiber optic light source with a frosted glass diffuser between the fiber and the chamber. 0-mL tuberculin syringe whose lateral location is controlled with a micromanipulator. Instead of a needle, a very fine tip is drawn from a microcapillary over a suitable flame and cut to provide a small Physical Properties of ATPS 2. 3. 4. 5. 6. 7. 8. 008 cm) for small drops. 0 cm are cut to carry or act as the test surfaces. Software: A standard image-analysis package, such as OPTIMAS™ is used to obtain the drop profile.

This facilitates the removal of portions of one stock solution in case of error during weighing. 45 g of buffer. Because of the problem of accurately pipetting the polymer stock solutions due to their high viscosity, they are measured by weight and are easily transferred using a Pasteur pipette with a broken tip. 4. The systems are shaken for 2–20 min by hand or eventually by using a rotary shaker (see Note 9). 5. The systems are permitted to settle for a period of 30 min up to 24 h depending on the system composition (see Note 10), or they are centrifuged for 2–15 min at 1500g.

Equilibration times vary with the system composition and type of surface. Most dextran/PEG systems will equilibrate in 30 min but drops can be left overnight to ensure complete equilibration, providing the temperature remains constant. 1 µL to ~1 µL, depending on the phase density difference, interfacial tension, viscosity and microcapillary syringe tip dimension. Larger drops deform too much to be analyzed accurately because of the low interfacial tensions. Drops that are essentially flat on top cannot be analyzed.

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Aqueous two-phase systems. Methods and protocols by Rajni Hatti-Kaul


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